| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Carducci, Austin J (11)
|
NITWST |
42.30 |
39.66 |
-2.64 |
| 2 |
Marks, Carter A (12)
|
NITWST |
56.83 |
42.48 |
-14.35 |
| 3 |
Venturo, Aidan J (12)
|
NITWST |
|
43.63 |
- |
| 4 |
Remick, Spencer S (12)
|
NIBAAC |
47.70 |
44.59 |
-3.11 |
| 5 |
Brauer, Jackson D (12)
|
NITWST |
49.02 |
44.95 |
-4.07 |
| 6 |
Suffoletto, Adam P (12)
|
NITWST |
50.19 |
46.13 |
-4.06 |
| 7 |
Hammer, Tristan J (11)
|
NISWAT |
1:00.25 |
47.17 |
-13.08 |
| 8 |
Molina Ayquipa, Santiago I (12)
|
NITWST |
1:01.96 |
48.77 |
-13.19 |
| 9 |
Lenz, Keegan C (11)
|
NITWST |
53.05 |
49.17 |
-3.88 |
| 10 |
Sippel, Patrick N (12)
|
NITWST |
55.75 |
49.19 |
-6.56 |
| 11 |
Pierce, Shilo J (11)
|
NIHAMB |
56.46 |
50.38 |
-6.08 |
| 12 |
Crone, Jayden P (11)
|
NISWAT |
1:01.50 |
52.42 |
-9.08 |
| 13 |
Schimert, William L (11)
|
NITWST |
|
54.16 |
- |
| 14 |
Nowicki, Kyle M (12)
|
NITWST |
|
56.43 |
- |
| 15 |
Powers, Jack N (11)
|
NIAMTS |
1:05.90 |
1:01.25 |
-4.65 |
|
Abram-Hopkins, Wesley A (11)
|
NIAAAC |
56.68 |
DQ |
- |
|
Lehnen, Cortland H (12)
|
NIHHSC |
|
DQ |
- |
|
Personius, Hunter J (12)
|
NIHAMB |
|
DQ |
- |
|
Bubar, Isaac J (12)
|
NITWST |
57.33 |
NS |
- |
|
Steiner, Ian M (11)
|
NITWST |
1:01.26 |
NS |
- |